his-tag protein problem

P.J. Shaw pjs14 at le.ac.uk
Tue Apr 18 10:07:04 EST 2000


I have purified a C-terminal His-tagged peptide on a His-bind 900
cartridge from Novagen. When I run it on SDS-PAGE, it runs at twice the
expected MW. I guess it's a dimer via a single S-S since there's a single
Cys residue. Is there a reducing agent stronger than DTT/B-ME which will
unzip it to monomers? 

Phil Shaw
Dept. of Genetics
Uni of Leicester, UK
pjs14 at le.ac.uk





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