Problems with GST fusion protein

Eva Chen yi-hui.chen at mcmail.vanderbilt.edu
Fri Apr 21 11:12:11 EST 2000


Is there anybody having experience on the purification of GST fusion
proteins?
Our lab doesn't have much experience and now there has been a serious
problem of degradation of the GST fusion protein. We have tried the
protease inhibitor from Sigma and Roche, and the whole purification
process was accomplished in a 4 C cold room. But the proteolysed
products are still there.

Any suggestions are highly appreciated. Thank you all for your
attention!

Eva Y. Chen
yi-hui.chen at mcmail.vanderbilt.edu
Jim Sutcliffe Lab
615-936-3627
Department of Molecular Physiology and Biophysics
Vanderbilt University School of Medicine
Nashville, TN 37232
USA





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