protein electrophoresis

Richard J. Dudley rdudley at pitt.edu
Wed Apr 26 08:11:06 EST 2000


If I read the question right, yes, the ab-ag will dissociate.  If you run a
Western, you'll see the heavy and light chains of the ab as the brightest bands
on your blot.

rich

Edward wrote:

> Hi, this might be a silly question, but I really need your help.
> I am using the product of immunoprecipitation for electrophoresis, that is,
> the samples are antibody-antigen complexes (is that right?).
> My question is that will there be a possiblity of degrading, or breaking of
> the ab-ag complexes, during the procedure of SDS-PAGE, which is a denaturing
> electrophoresis.
> Thanks in advance.
> Ed

--
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Cystic Fibrosis Research Center
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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