protein electrophoresis

Paul Klosen klosen at neurochem.u-strasbg.fr
Wed Apr 26 09:33:58 EST 2000



Edward wrote:
> 
> Hi, this might be a silly question, but I really need your help.
> I am using the product of immunoprecipitation for electrophoresis, that is,
> the samples are antibody-antigen complexes (is that right?).
> My question is that will there be a possiblity of degrading, or breaking of
> the ab-ag complexes, during the procedure of SDS-PAGE, which is a denaturing
> electrophoresis.
> Thanks in advance.
> Ed

Denaturing SDS-PAGE will most certainly dissociate ab-ag complexes. If
your sample buffer includes beta-mercaptoethanol or dithiotreitol, it
will even separate your antibodies into light and heavy chains. SDS-PAGE
after immunoprecipitation is not normally used to analyse the complexes
themselves, but rather to identify the subunits of these complexes.

Paul
-- 
Paul Klosen, PhD
CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
Universite Louis Pasteur  12, rue de l'Universite F-67000 Strasbourg,
FRANCE
Tel. 03.88.35.85.04  Fax. 03.88.24.04.61  klosen at neurochem.u-strasbg.fr




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