protein electrophoresis

Hiranya S. Roychowdhury hroychow at nmsu.edu
Wed Apr 26 09:44:07 EST 2000


Yes, they would, since the ab-ag complex is not covalent.

At 06:11 PM 4/25/00 -0700, Edward wrote:
>Hi, this might be a silly question, but I really need your help.
>I am using the product of immunoprecipitation for electrophoresis, that is,
>the samples are antibody-antigen complexes (is that right?).
>My question is that will there be a possiblity of degrading, or breaking of
>the ab-ag complexes, during the procedure of SDS-PAGE, which is a denaturing
>electrophoresis.
>Thanks in advance.
>Ed
>
>
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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