protein purification and aggregation

Alexander xander.drg at gmx.net
Tue Aug 8 05:00:09 EST 2000



Frank Fuerst schrieb:

> Alexander wrote:
> >
> > bands on SDS-PAGE. I also noticed an increasing pressure of the column.
> > Summarized I got two results, either the proteins appeared in the void volume
> > or they disappeard. Thus my interpretation is that the proteins form aggregats
> > which either stick to the column or do not interact at all. It is confusing.
> >     But I have no direct proof for the formation of aggregats. What else could
> > it be?
>
> I also think that it is aggregation.
>
> Have you tried working in the cold room?
>
> Frank

Yes, I started to work at 4°C to minimize degradation (using buffer with PMSF,
Leupeptin, Pepstatin, Antipain), but it did not help.

Alexander






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