DTNB-modification of proteins

Paul S. Brookes. brookes at uab.edu
Mon Aug 14 11:21:55 EST 2000


Dialysis always takes too long IMHO.   Assuming your protein doesn't bind 
GSH, it might be possible to remove it much faster by running your sample 
down a GSH-sepharose column, like those used for purifying GST-fusion proteins.

Regards
PSB

_________________________________________
Dr. Paul S. Brookes.            (brookes at uab.edu)
UAB Department of Pathology,   G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915     Fax (001) 205 934 1775
http://peir.path.uab.edu/brookes

The quality of e-mails can go down as well as up
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