removal of SDS from protein samples

[Qishan Lin]

How about using hydroxylapatite coulmn?
Good luck!

Qishan
HHMI


On Thu, 27 Jul 2000, Kresten wrote:

> 
> > Does anyone know of an easy way to removal SDS from protein samples.
> 
> There is something about cyclodextrin (cannot remember which one) binds
> SDS strongly. Has been used to study refolding of SDS denatured
> proteins. A quick medline gave:
> 
> Couthon F, Clottes E, Vial C
> Refolding of SDS- and thermally denatured MM-creatine kinase using
> cyclodextrins.
> Biochem Biophys Res Commun 1996 Oct 23;227(3):854-60
> 
> but I am sure there is other stuff as well. Perhaps sci.chem can help.
> 
> I guess you have to distinguish between removing SDS from solution and
> from your protein. Although removing most SDS from the solution
> (chromatography, precipitation) will draw some SDS away from your
> protein I am not sure that all the strongly bound SDS can be easily
> removed these ways.
> 
> If you find a good (and easy) way, could you not post it, just for the
> record?
> 
> HTH
> Kresten
> 
> --
> The address kresten at my-dejanews.com is for
> spambots only. Please mail me at LysLeuLeu at crc.dk
> transforming the pre at -part into my initials.
> Kresten Lindorff Larsen
> 
> 
> Sent via Deja.com http://www.deja.com/
> Before you buy.
>