removal of SDS from protein samples
Achim Recktenwald, PhD
ARecktenwald at StressGen.com
Tue Aug 22 18:13:00 EST 2000
The best method know of and the one used by myself is precipitation in very
cold (< -20C) acetone. SDS dissolves in the acetone.
The acetone has to be at least at -20C, otherwise either your protein won't
precipitate or denature. You have to cool down all vessels and other items
that come into contact with the cooled acetone.
If your protein binds SDS very strongly, repeat the procedure.
This is in my opinion the best method, but it is almost impossible to remove
ALL the SDS from protein.
"Qishan Lin" <lqs at u.washington.edu> wrote in message
news:Pine.A41.4.21.0008161459550.95146-100000 at homer08.u.washington.edu...
> How about using hydroxylapatite coulmn?
> Good luck!
> On Thu, 27 Jul 2000, Kresten wrote:
> > > Does anyone know of an easy way to removal SDS from protein samples.
> > There is something about cyclodextrin (cannot remember which one) binds
> > SDS strongly. Has been used to study refolding of SDS denatured
> > proteins. A quick medline gave:
> > Couthon F, Clottes E, Vial C
> > Refolding of SDS- and thermally denatured MM-creatine kinase using
> > cyclodextrins.
> > Biochem Biophys Res Commun 1996 Oct 23;227(3):854-60
> > but I am sure there is other stuff as well. Perhaps sci.chem can help.
> > I guess you have to distinguish between removing SDS from solution and
> > from your protein. Although removing most SDS from the solution
> > (chromatography, precipitation) will draw some SDS away from your
> > protein I am not sure that all the strongly bound SDS can be easily
> > removed these ways.
> > If you find a good (and easy) way, could you not post it, just for the
> > record?
> > HTH
> > Kresten
> > --
> > The address kresten at my-dejanews.com is for
> > spambots only. Please mail me at LysLeuLeu at crc.dk
> > transforming the pre at -part into my initials.
> > Kresten Lindorff Larsen
> > Sent via Deja.com http://www.deja.com/
> > Before you buy.
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