reversible protein staining

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Dec 4 05:53:49 EST 2000


In article <90946s$hqq$2 at news.doit.wisc.edu>, the eminent Dima Klenchin
at UW wrote
>"My" protein binds very poorly and to elute even at 10 mM
>imidazole. No problems - I just load without any, wash extensively
>with 5 mM, briefly with 10 mM, elute with bump of 100 mM 

Are you using Ni or a Co charged column? For some proteins we found that
Co is way better than Ni for binding etc.

Maybe worth a go.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






More information about the Proteins mailing list