reversible protein staining
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Mon Dec 4 05:53:49 EST 2000
In article <90946s$hqq$2 at news.doit.wisc.edu>, the eminent Dima Klenchin
at UW wrote
>"My" protein binds very poorly and to elute even at 10 mM
>imidazole. No problems - I just load without any, wash extensively
>with 5 mM, briefly with 10 mM, elute with bump of 100 mM
Are you using Ni or a Co charged column? For some proteins we found that
Co is way better than Ni for binding etc.
Maybe worth a go.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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