Western problem

huangz123 at my-deja.com huangz123 at my-deja.com
Mon Dec 4 07:13:38 EST 2000


Did you check the buffer? Sometimes when the buffer in upper reserviour
is low it will cause discontinuous of the electric current.

zhong huang
http://biowww.net

In article <Pine.A41.4.21.0011291118340.125468-
100000 at dante41.u.washington.edu>,
  "D. Diaz" <dolo at u.washington.edu> wrote:
> This is a weird one:
>
> Lately when running my SDS-PAGE gels my proteins get stuck at about 1
cm
> from the bottom of the gels, they just don't go any further and they
pile
> up in there; the marker also gets stuck but a little bit of it goes
> through.
>
> Any ideas?
>
> ***********************************************
> Dolo Diaz				Phone: (206)-616-2645
> Department of Environmental Health	Fax: (206)- 685-4696
> University of Washington		E-mail: dolo at u.washington.edu
> 4225 Roosevelt Way NE, suite #100
> Seattle, WA, 98105
>
>


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