easy elution from acrylamid gel?

[Natalia Battchikova]

Hello, Aldert,

Is this method possible for silver-stained proteins? What is your estimation
of the elution efficiency?

Regards
Natalia

Aldert Zomer wrote:

> "M. Hertel" wrote:
> >
> > Hello
> > we are looking for a low tech, handmade method for eluting a Protein out
> > of an acrylamid gel band. Nothing where You need an electroeluter or
> > columns etc. but something simple like dialysis, elution in a agarosegel
> > chamber , boiling in a buffer???
> > thanks for Ideas
> > Moritz
>
> Cut out the band with the protein, place it in a suitable dialysis tube
> (as small as possible) with the appropriate buffer, and place this in
> the electrophoresis chamber for agarose gels (+buffer) and start
> electrophoresis. The protein will go out the gel but remain in the
> dialysis tube.
>
> --
> Ing. Aldert Zomer,                      | Tel: +31 50 36 32 360
> Research Technician,                    | Fax: +31 50 36 35 205
> IMEnz Bioengineering,                   | Email: zomeral at biol.rug.nl
> PO BOX 14, 9750 AA Haren, Netherlands   | Homepage: http://www.imenz.com
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--
Dr. Natalia Battchikova

University of Turku, Biology dept.
Lab. of Plant Physiology and Molecular Biology
BioCity Tykistokatu 6A
FIN-20520 Turku
Finland
tel. 358-2-3338078
fax: 358-2-3338075
natbat at utu.fi