Slimy cell suspensions after lambda CE6 induction

Emir ekhatipo at NOSPAMmidway.uchicago.edu
Thu Dec 7 13:25:21 EST 2000


I was inducing expression of a protein from a T7-type (pET11-based)
construct in E.coli BLR cells with bacteriophage lambda CE6 that delivers T7
polymeraze into the bacterial cell upon infection. I noticed that at a
ration of phage to cells more than ~10 the frozen and then thawed and
resuspended in a neutral buffer pellet would exhibit higher viscosity that
non-phage induced cells, i.e., cells look lysed and the suspension is rather
slimy. Does anybody have an explanation of this phenomenon? Lambda CE6 is
not supposed to lyse BLR cells, and just lysogenizes them.

Emir







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