blurred bands in coomassie staining?

Arnoud van Vliet avvliet at knoware.nl
Thu Dec 7 13:35:37 EST 2000


> what am I doing wrong if the bands of a coomassie-stained SDS-PAGE gel
> look rather blurred and fuzzy?
> Sample preparation was:
> Pellet from 1ml Bakteria, resuspended in 140ul 1xSDS sample buffer,
> heated to 100deg 5min. 40ul were loaded.

Martin,

possibly the medium used to grow the bacteria. Wash the cell pellet once
with PBS, and then resuspend in sample buffer. Also, load less protein, as
you seem to be loading a lot.

hope this helps
Arnoud








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