blurred bands in coomassie staining?

Emir ekhatipo at NOSPAMmidway.uchicago.edu
Fri Dec 8 11:41:00 EST 2000


You are definitely overloading. I don't know what the OD of your cells was,
but for the cells at OD~1 I would take as little as 5ul of the lysate
prepared as you described.

Emir


"Martin Hoehne" <marthoe at biologie.uni-freiburg.de> wrote in message
news:3A2F58CA.CD5AB557 at biologie.uni-freiburg.de...
> Hello,
>
> what am I doing wrong if the bands of a coomassie-stained SDS-PAGE gel
> look rather blurred and fuzzy?
> Sample preparation was:
> Pellet from 1ml Bakteria, resuspended in 140ul 1xSDS sample buffer,
> heated to 100deg 5min. 40ul were loaded.
>
> Any hints?
>
> Thanks,
>
> Martin
>
> --
> ________________________________________
>  Martin Hoehne
>  Institut fuer Biologie III
>  Labor Prof. Fischbach
>  Schaenzlestr. 1
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>
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