blurred bands in coomassie staining?
ekhatipo at NOSPAMmidway.uchicago.edu
Fri Dec 8 11:41:00 EST 2000
You are definitely overloading. I don't know what the OD of your cells was,
but for the cells at OD~1 I would take as little as 5ul of the lysate
prepared as you described.
"Martin Hoehne" <marthoe at biologie.uni-freiburg.de> wrote in message
news:3A2F58CA.CD5AB557 at biologie.uni-freiburg.de...
> what am I doing wrong if the bands of a coomassie-stained SDS-PAGE gel
> look rather blurred and fuzzy?
> Sample preparation was:
> Pellet from 1ml Bakteria, resuspended in 140ul 1xSDS sample buffer,
> heated to 100deg 5min. 40ul were loaded.
> Any hints?
> Martin Hoehne
> Institut fuer Biologie III
> Labor Prof. Fischbach
> Schaenzlestr. 1
> 79104 Freiburg
> Tel +49-(0)761-203-2759
> Fax +49-(0)761-203-2866
> Email marthoe at biologie.uni-freiburg.de
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