Protein A chromatography

Bruce Coryell brucec at brucec.mv.com
Mon Dec 11 15:14:11 EST 2000


Does anyone have experience with cleaning and regenerating a Perseptive
Biosystems Protein A cartridge.  I  have a Protein A cartridge that is
badly fouled and generating high back pressures, but no money in budget
for another one.   Any  hope?  Obviously any cleaning method would need
to not denature or cleave the Protein A ligands...






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