GST-Ab problems

pmoreno pmoreno at servidor.ibv.csic.es
Mon Dec 18 07:42:07 EST 2000


I´m using the GST-antibody to detect by western blot a protein with this
fusion protein from a extract of E.coli. My problem is when I make a
SDS-gel and after I tranfer to membrane ( PVDF) and detect with the Ab (
from Santa.Cruz) I see a lot of bands. What can I do?. Because I use
this method to detect the espression of my protein but it is
imposible.The Ab primary is 1:1000 and the secondary 1:5000.






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