PMSF dire straits

BHKim at
Tue Feb 1 06:21:34 EST 2000

"Frank O. Fackelmayer" schrieb:

> HOWEVER, stability of PMSF is hardly ever a concern because its mode of action.
> It covalently modifies the active center of many (serine) proteases, and makes
> them proteolytically inactive. This modification is fast, and once it happened it
> is irreversible, so you really donut have to care about breakdown of the excess
> PMSF afterwards.

> Frank

I'm not sure, but I've heared that it can be reversible when there is reducing power
(like DTT) in the solution.


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