PMSF dire straits

Colin Rasmussen colin at pombe.usask.ca
Tue Feb 1 16:58:01 EST 2000



simm wrote:

> Dear Protein experts,
>
> I am currently running experiments requiring the use of PMSF. I have to
> prepare fresh
> solutions each time which as you may know takes up time and wastes a lot of
> the
> reagent. Is there a way to prepare a solution which is stable in storage?
> are there other similar protease inhibitors? I am specifically interested in
> stability of PMSF in methanol,  acetone and isopropanol over time. I have
> heard some colleagues store such solutions for up to a month, but I need
> confirmation from the scientific body.

We make concentrated stock in isopropanol.  They store just fine.

Colin





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