PMSF dire straits

bradley turner turner at sprcore.caregroup.harvard.edu
Tue Feb 1 21:42:49 EST 2000


Hello Margaret,

I've always prepared 1.0M stock solutions of PMSF in DMSO
and stored aliquots at -20oC.  I've used them for years.

Hope this helps,
Brad Turner

****************************************************************
                    Bradley Turner
                Beth Israel Deaconess
                    Medical Center

Harvard Medical School          617-667-1215 phone
Division of Gastroenterology    617-667-2767 fax
Room Dana 536                   bsturner at biosun.harvard.edu
330 Brookline Avenue            bturner at caregroup.harvard.edu
Boston, MA 02215                turner at sprcore.caregroup.harvard.edu
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On Fri, 28 Jan 2000, simm wrote:

> Dear Protein experts,
> 
> I am currently running experiments requiring the use of PMSF. I have to
> prepare fresh
> solutions each time which as you may know takes up time and wastes a lot of
> the
> reagent. Is there a way to prepare a solution which is stable in storage?
> are there other similar protease inhibitors? I am specifically interested in
> stability of PMSF in methanol,  acetone and isopropanol over time. I have
> heard some colleagues store such solutions for up to a month, but I need
> confirmation from the scientific body.
> 
> Kindly, please respond to my email address:
> simm at worldnet.att.net
> 
> Best regards,
> 
> Dr. Margaret Simm, PhD
> 
> 
> 

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