stripping nitrocellulose membrane

[Ilya Shamovsky]

Sandra Pena wrote:

> Can somebody give me a good protocol for stripping nitrocellulose
> membranes for immunoblots?
> Please send it to me at: sandra_vazquez at hotmail.com
>
> ---

Sandra,

Try 50 mM glycine, pH 2.3 + 1 mM EDTA for 15-30 min followed by PBS/0.1%
Tween 20 and then reprobing the membrane as usual. Works great for me.

Alternatively, 30 min at 50-70 degrees C in 62.5 mM tris-Cl, pH 6.8, 100
mM beta-mercaptoethanol and 2% SDS should do the job.

Hope this helps.
Cheers,
Ilya.