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acid phosphatase

Frank Fuerst fant.1 at gmx.net
Mon Feb 7 04:28:59 EST 2000


sandy_globin at my-deja.com wrote:
> 
> 1. could someone please define specific enzyme activity, and also give
> its formula?

specific catalytic activity is the activity of a enzyme (as measured
by an assay that is, hopefully, specific for that one enzyme) divided
by the mass of protein in the sample. Thus, it is a measurement not
only for the "intrinsic" activity (that could by measured by the molar
activity), but also for the purity with respect to other proteins or
peptides.

The formula is (I'm using z as the sign for catalytic activity)

z_sp(enzyme) = z(enzyme)/m(protein), where

z(enzyme) = Delta_n(substrate)/Delta_t

where Delta_n(substrate) is the change in amount of substance (in
moles) of substrate (or product), as measured in the assay, in the
time Delta_t.

> 2. where can i get the Km value for wheat germ acid phosphatase?

I'm sorry.

Frank
-- 
Flhacs wird im Usenet grundsätzlich alsfhc geschrieben. Schreibt man
lafhsc nicht slfach, so ist das schlichtweg hclafs. Hingegen darf man
rihctig ruhig rhitcgi schreiben, weil eine shcalfe Schreibweise bei
irhictg nicht als shflac angesehen wird. [Hajo Pflüger in dnq]




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