On 4 Feb 2000, Sandra Pena wrote:
> Date: 4 Feb 2000 19:40:29 -0000
> From: Sandra Pena <spena at upracd.upr.clu.edu>
> Newsgroups: bionet.molbio.proteins
> Subject: stripping nitrocellulose membrane
>> Can somebody give me a good protocol for stripping nitrocellulose
> membranes for immunoblots?
> Please send it to me at: sandra_vazquez at hotmail.com>> ---
How about this one:
Incubate your membranes in 0.2 M NaOH for 5 min. Neutralize in TPBS or
TTBS (Tween - PBS etc.) and block with 5% milk in ditto. I think this is a
modification of protocol from Red Book, it works fine for me (might be a
little rough, though).
Pavel Sova, Ph.D.
ps44 at columbia.edu