Purification of monomeric actin

Richard P. Grant rgrant at netscape.net
Tue Feb 8 10:52:58 EST 2000


In article <4txweIA4sCo4EAMY at genesys.demon.co.uk>, "Dr. Duncan Clark" 
<Duncan at genesys.demon.co.uk> wrote:

> Brilliant! Just what I was after.

<bows>  pleased to be of service.

> Now for the bunnies or should I juts
> go and get a few lb of primer beef steak :-). I don't suppose for the
> use I'm going to put it too that it will matter.
> 

Hmm, any skeletal muscle should do.  Bunnies, cute, white, fluffy white 
New Zealand bunnies are traditional because animal houses usually have a 
surfeit of retired, fat, old animals, and it's good practice to take the 
muscle when an animal is terminally bled for antisera.

It seems a waste of prime steak to make actin from it.   :))

You could use beef heart[0] I guess, but then you'd get smooth muscle 
actin . . .

R

(who is intrigued - why do you want grams of G-actin?)

[0] Damn.  None of us here can remember what Captn Beefheart's hit was!

-- 
Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
Please reply to rpg 'at' mrc-lmb.cam.ac.uk




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