BL21(DE3) and pTrc

DOCTORHIM at aol.com DOCTORHIM at aol.com
Fri Feb 11 23:42:50 EST 2000


That is complete BS.  Adding additional lac repressor will just repress the 
T7 polymerase more.  You might have a problem transforming with the lac 
operator.  This binding sequence may titrate out the repressor inducing the 
polymerase.  Even then,  the cells will live fine.  Even if you induce the 
polymerase and have a T7 promoter with no translatable gene attached,  the 
cells will live fine.  Lethality occurs only if you induce the T7 RNA 
polymerase and have a T7 promoter transcribing a translatable gene.   
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