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Coomassie stain on Western Blots

Thomas thomas.korosecNOthSPAM at univie.ac.at.invalid
Mon Feb 14 03:48:02 EST 2000


The effect you are both observing, must have something to do
with the type of membrane used. Using nitrocellulose
membranes you will always get a perfectly red membrane, when
you use Ponceau S AFTER blocking with low fat milk powder in
TBS or BSA or whatsoever and after a "full" Western blot, of
course.


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