32P gel developping

Paola Furla paola.furla at physiol.ox.ac.uk
Thu Feb 17 09:02:11 EST 2000


Dear M. or Miss,
I need to develop a SDS-polyacrylamide gel containing proteins labeled
with 32P, I would like to know what do you suggest me to use as X-Ray
films and intensifying screens.
Moreover, I thought to dry the gel before develop it, but I read about a
method which consists to freeze the gel(-20 C or -70 C ?) before to use
it. What could you tell me about that? If I freeze the gel, could I also
use it for immoblot?  
Thank you very much in advance.
Paola Furla
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Dr. Paola Furla
University Laboratory of Physiology, Oxford, UK
mailto:paola.furla at physiol.ox.ac.uk




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