nickel concentration or chelation

Arnoud van Vliet avvliet at knoware.nl
Wed Jan 5 16:49:25 EST 2000


Hi,

does anybody know the concentration of nickel in Brucella broth or other
(comparable) microbiological media? Or a protocol how to measure it, without
specific equipment; a colorimetric assay, preferably. My searches in the
Medline only resulted in a xylenol orange assay, but that's not specific for
nickel.

While I am at it, does anybody know a nickel specific chelator, so I can
measure the effect of varying the nickel conc on my pet microbe?

Thanks in advance,
Arnoud

--
Dr. A.H.M. van Vliet
Vrije Universiteit
Amsterdam
The Netherlands

email: avvliet at knoware.nl









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