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His-tag protein purification

Neil McKenna nmckenna at ottawa.com
Thu Jan 6 10:13:33 EST 2000


Try http://www.qiagen.com/literature/handbooks/qxp/qxp/qxp.pdf

It is their "qiaexpressionist" handbook for use with their Ni-NTA
column. I have no affiliation with them, but this handbook is excellent
for protocols and troubleshooting. 

Yeyan Zhang wrote:
> 
> Hi, Everyone:
> 
> I am looking for a protocol that can purify his-tag proteins under denatured
> conditions, preferably with his-tag columns. Any info will be highly
> appreciated.
> 
> -Yeyan
> ______________________________________________________
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> 
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