pfu and taq PCR mutation percentage

Liu Xiangyu liuxiangyu at hotmail.com
Fri Jan 7 23:27:57 EST 2000


Hi, All, I am constructing a expression vecter and having a question
about fidelity
                 comparison of Taq and pfu polymerase
for Taq: error rate is 8.0 X 10^-6   For pfu: ..... is 1.3 X 10^-6
                 According to stratagene pfu DNA polymerase manual, the
percentage of mutated PCR products after amplification of a 1KB target
seq for 20 cycles are 2.6% for pfu and 16.0% for Taq.
                 Does anybody knows a simple formula to caculate? for
example, what happens to 3 KB target after 10 cycles by pfu or taq?
                 If I use Taq for 10 cycles and pfu for 20 cycles( since
pfu always give much weak  bands), which one gives better result
considering percentage of mutation.
                Thank you for your help.
                 Liu





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