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Ethanol-chloroform, need help

Sylvain Kuony skuony at club-internet.fr
Sun Jan 16 15:57:41 EST 2000


I use a 1:1 ratio of EtOH-chloroform
. Do you know what could explain the loss of my protein?
S. Kuony
rwn <rwnNOrwSPAM at mbio.aau.dk.invalid> a écrit dans le message :
13001ab4.2578afb6 at usw-ex0108-062.remarq.com...
> What is your ratio of EtOH:chloroform?
>
> I use a 2.5 vol excess of EtOH when precipitating protein
> from a phenol phase. All at room temperature.
>
> RWN
>
>
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