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Ethanol-chloroform, need help

rwn rwnNOrwSPAM at mbio.aau.dk.invalid
Sat Jan 15 19:13:40 EST 2000


Well, this is not my strongest side, but I believe that your
protein could simply be soluble in the chloroform phase
(like I use a phenol phase to extract protein).

Unless you get better suggestions I would start by adding
more EtOH. If you have enough protein, you can actually see
it precipitate out at the critical EtOH vol.

good luck, rwn



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