His-tag protein purification

Chris Larosa clarosa at biocomp.unl.edu
Mon Jan 17 14:56:05 EST 2000


Just go to the Qiagen web site and download the qiaexpressionist hand book.
You can download all you need from their web pages on his-tagged proteins.
Their reagents worked great for producing a fusion for antibody production for
me.
PCPhD

Yeyan Zhang wrote:

> Hi, Everyone:
>
> I am looking for a protocol that can purify his-tag proteins under denatured
> conditions, preferably with his-tag columns. Any info will be highly
> appreciated.
>
> -Yeyan
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