Just go to the Qiagen web site and download the qiaexpressionist hand book.
You can download all you need from their web pages on his-tagged proteins.
Their reagents worked great for producing a fusion for antibody production for
Yeyan Zhang wrote:
> Hi, Everyone:
>> I am looking for a protocol that can purify his-tag proteins under denatured
> conditions, preferably with his-tag columns. Any info will be highly
> Get Your Private, Free Email at http://www.hotmail.com>> ---