Hydrophobic interaction chromatography problem

Peeter Toomik peeter.toomik at ut.ee
Thu Jan 20 06:18:26 EST 2000


If you want to prepare a sorbent with the structure like Phenyl- or Octyl
Sepharose then mix cross-linked Sepharose with glycidyl propyl ether in
anhydrous dioxane, and add few drops of boron trifluoride. The reaction is fast
and almost quantitative. I am afraid that you must synthesize the ether by
yourself from epichlorohydrine and propanol.
If you bind propylamine onto any activated matrix, you will get a sorbent that
is unstable and/or also able to bind some proteins by other interactions than
hydrophobic.

Peeter Toomik


Lars Komorowski wrote:

> I want to use a propyl residue as the active group. My problem is that only
> Sigma seems to vend some old stuff based on agarose as  matrix. I want to
> know if there are some other possibilities.
> Another solution is to manufacture the material by myself. Has someone got a
> recipe for doing it (needed reagents, activated groups on the matrix etc.) ?
> Lars





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