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Hydrophobic interaction chromatography problem

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Thu Jan 20 15:28:14 EST 2000


:I want to use a propyl residue as the active group. My problem is that only
:Sigma seems to vend some old stuff based on agarose as  matrix. I want to
:know if there are some other possibilities.
:Another solution is to manufacture the material by myself. Has someone got a
:recipe for doing it (needed reagents, activated groups on the matrix etc.) ?
:Lars
:
Unless propyl is terribly essential (which it isn't probably), you can try Bio-Rad's
"Macro Prep" metacrylate-based supports. They come as methyl and t-butil
derivatives. The matrix is exceptionally good, IMHO: rigid, packs 
extremely well, withstands high pressures and harsh regeneration 
conditions (yet can be used with LPC systems) and not deteriorated by air 
as agarose. They also sell activated matrices to which you always couple 
propylamine to get exactly what you want. The beads are ~ 2X smaller than
typical cross-linked agarose so you get slightly better resolution, too.

I used their Macro Prep DEAE and Q and was very happy with them. 

        - Dima




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