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Ethanol-chloroform, need help

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Mon Jan 24 08:42:39 EST 2000


Are you sure your protein will precipitate with EtOH/CHCl3?
If not, do a couple of precipitations in parallel and see which one is
most effective.
things to try:
- EtOH/CHCl3
- EtOH (4 vol.)
- acetone (4 vol.)
- isopropanol (4 vol.)
- and of course old-fashioned TCA and ammonium sulphate.
Good luck,

Rogier
Dept MicFizz
Free U of A
Amsterdam


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