thermostable proteases

[Kresten]

I am looking for a thermostable protease preferably working around pH 5.
Does anybody know any proteases fulfilling these requirements? I do not
need specificity, actually the less specific the better.

With regards to the temperature range, the bigger the better is my
answer at the moment although I might be satisfied by a protease working
around 70 degreesC. Also it would be nice if the protease was
commercially available.

Does anybody know how low a value of pH one can use thermolysin in and
still get reasonable activity?

thank you
Kresten

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Kresten Lindorff Larsen, Dept. Yeast Genetics


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