In vivo chemical-crosslink??

Byung-Hoon Kim byung-hoon.kim at uni-tuebingen.de
Wed Jul 5 09:59:53 EST 2000



Martin Offterdinger schrieb:

> Hi netters,
> Is it possible to do in vivo chemical crosslinking, i.e. treat cells with a
> crosslinker and isolate proteins afterwards in order to analyze protein
> interactions via immunopreciptation and/or Western?
> Which crosslinker (s) can be used for that purpose?
> Which controls should be done?
> Any input is highly appreciated!
> Thank you!
> martin

You can find such experment in  <Cell 94: 471-480 (1998)>.
They used DSP to corss-link interacting proteins in vivo.


Byung-Hoon Kim

ZMBP-Allgemeine Genetik
Uni Tuebingen






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