removal of SDS from protein samples

MArk Bond Mark.bond at bris.ac.uk
Mon Jul 10 06:40:22 EST 2000


Hi,

Does anyone know of an easy way to removal SDS from protein samples. I know
you can get detergent out columns from genotech that remove free sds but how
can i remove SDS bound to the protein?  Can I add triton - x- 100 ? This
should form micells and pull the SDS from the protein into the hydrophobic
micells, shouldn't it? Can I do enzyme assays with the triton there?

Hope you can help

Please reply to my e-mail address = Mark.bond at bris.ac.uk


Cheers

Mark Bond







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