How to force recombinant proteins to get into a tetrameric form?

Dirk Seegert D.Seegert at mucosa.de
Tue Jul 11 11:08:52 EST 2000


Hi there,
I wonder wheather anybody could give me hints on how to get a monomeric
recombinant protein into its natural occuring bioactive tetrameric form. Is
it possible to solve this problem by production of tagged proteins? If so,
which tags could be useful?  Do I have to use specific cell lines?
I would be happy for any advice.

Thanx,
Dirk Seegert


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Dr. Dirk Seegert
Christian-Albrechts-Universität Kiel
1. Medizinische Klinik, Mucosaimmunologie
Schittenhelmstr. 12
D-24105 Kiel
GERMANY

Tel. +49 (431) 597-1373
Fax +49 (431) 597-1842
http://www.mucosa.de




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