removal of SDS from protein samples

[Bernard Murray, PhD]

In article <FxHCFA.HCC at bath.ac.uk>, "MArk Bond" <Mark.bond at bris.ac.uk> wrote:

> Does anyone know of an easy way to removal SDS from protein samples. I know
> you can get detergent out columns from genotech that remove free sds but how
> can i remove SDS bound to the protein?  Can I add triton - x- 100 ? This
> should form micells and pull the SDS from the protein into the hydrophobic
> micells, shouldn't it? Can I do enzyme assays with the triton there?

I believe there are protocols involving passage through XAD-2 (or similar)
columns.  A non-denaturing protocol will only reduce the SDS concentration
in solution.  Any SDS that sticks to protein itself requires very harsh
conditions to remove it.

> Please reply to my e-mail address = Mark.bond at bris.ac.uk

No.

   Bernard

-- 
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF