removal of SDS from protein samples

Robert.Goodman at godi.adirondack.fidonet.org Robert.Goodman at godi.adirondack.fidonet.org
Tue Jul 11 23:43:00 EST 2000


reply-to: robgood at bestweb.net
  "MArk Bond" <Mark.bond at bris.ac.uk> wrote:
> Hi,
>
> Does anyone know of an easy way to removal SDS from protein samples. I know
> you can get detergent out columns from genotech that remove free sds but how
> can i remove SDS bound to the protein?  Can I add triton - x- 100 ? This
> should form micells and pull the SDS from the protein into the hydrophobic
> micells, shouldn't it? Can I do enzyme assays with the triton there?

I'd try dialysis or one of those membrane-type devices that substitute for
dialysis.


Sent via Deja.com http://www.deja.com/
Before you buy.






More information about the Proteins mailing list