removal of SDS from protein samples

Rogier rogierNOroSPAM at bio.vu.nl.invalid
Wed Jul 12 08:23:21 EST 2000


removal of SDS from protein solutions:

equilibrate QAE-sephadex in 50 mM NaPi pH7, 200 mM NaCl.

make a column (in a syringe, pipet,whatever)

load protein solution, elute with 50 mM NaPi pH 7, 200 mM NaCl

protein comes out, SDS stays in the column

Good luck,
Rogier



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