removal of SDS from protein samples
Paul S. Brookes.
brookes at uab.edu
Wed Jul 12 13:31:45 EST 2000
Try "biobeads" made by BioRad (no affiliation). They worked in our hands
for removing both triton and deoxycholate off a protein. However, loss of
detergent did cause the protein ( amembrane protein) to lose its activity -
I suppose it depends on your protein and what you want to do with it
afterwards? Not sure if the beads work for SDS.
Regards
_________________________________________
Dr. Paul S. Brookes. (brookes at uab.edu)
UAB Department of Pathology, G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915 Fax (001) 205 934 1775
http://peir.path.uab.edu/brookes
The quality of e-mails can go down as well as up
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://iubio.bio.indiana.edu/bionet/mm/proteins/attachments/20000712/4fe8a6fb/attachment.html
More information about the Proteins
mailing list