Alternate to CHAPS detergent

news.vtacs.com lcomaratta at croptech.com
Tue Jul 25 09:49:19 EST 2000


I am looking for an alternate to using CHAPS.  The buffer that I use is:

20 mM HEPES, pH 7.4
12.5 mM CHAPS
1M Guanidine-HCl
1mM EDTA

It is used to secrete proteins from plant tissue.  The problem that I am
facing is that this detergent seems to be getting more and more difficult to
obtain.  Many of our regular suppliers are on constant backorder.  Also, the
price is rather high.

I am looking for an alternative compound to use in place of CHAPS.  If
anyone has any information, I would be most appreciative.

Please send any information to:

lcomaratta at croptech.com

Thank you.

Len Comaratta







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