Problems with Protein Expression

jkuruvil jkuruvil at lamar.colostate.edu
Wed Jul 26 17:58:34 EST 2000


Hi All,

I donot know if  this is the right group to place this question ...!! but
..does anyone have any experience in solubilizing a protein ..found in
large amounts..in a Sf-9 cell line Pellet?? Molecular wt is around 60kD
..and..is estimated to be expressed at high conc...(150mg/lit) ...The
protein was harvested from  large plates as well as a spinner flask 48 hrs
post infection.

Any help is higly appreciated

Thanks in advance.....


Jes








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