large scale deglycosylation

bocola at mailer.uni-marburg.de bocola at mailer.uni-marburg.de
Mon Jul 31 10:44:41 EST 2000


I would like to deglycosylate approx. 10 mg Protein for crystallisation.

The glycan is mannose-rich and Asn-N-linked.

Does anyone have experiences with native deglycosilation ?
(Most protocols, supplied with PNGase F or endo H are only for
analytical
purposes , max 100 µg for SDS-analysis under denaturating conditions)

ANY HINTS APPRECIATED, thanks in advance !

bye , Marco

--
Marco Bocola   Dept. of pharmaz. Chemistry
    AG Prof. Klebe
Tel: +49-6421-28-5071  Marbacher Weg 6
Fax:            -8994  D-35032 Marburg

http://pc1664.pharmazie.uni-marburg.de/agklebe
e-mail:bocola at mailer.uni-marburg.de

:-( There are no problems, just solutions !? :-)







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