Pierce "gentle elution buffer"

Roger Murphy roger.murphy at ludwig.edu.au
Tue Jun 13 23:35:34 EST 2000


A question for the assembled throng....

We're going to use the Pierce "gentle elution buffer" to elute an
acid-sensitive monoclonal antibody from a protein A column.  The Pierce
instructions warn of dire precipitation consequences if this buffer ever
comes into contact with phosphate salts...

Does anyone know why?  What is the ingredient in their buffer that will
precipitate with phosphates?

Thanks for your anticipated help!

Roger






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