prokaryotic expression systems??

Paul D. Guthrie guthrie at Hub.TCH.Harvard.edu
Fri Jun 16 13:59:55 EST 2000


We're looking to express and purify a human protein of about 120 amino
acids in E. coli. Does anyone have any feelings, one way or the other,
about the following systems?

    Clontech pPROTet.E

    Promega PinPoint Xa

    Stratagene pCAL

The Stratagene system looks pretty attractive, but we'd like to hear
from someone who's actually used one or all of these. Thanks for any
advice!!


Paul D. Guthrie
Urology Research Lab
Children's Hospital, Boston




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