Help with Taxol preps

Centriole centriole at home.com
Mon Jun 26 22:23:23 EST 2000


I got the same thing happening with Nocodazole.  At first I thought my cells
were contaminated.  The interesting thing is, it only seems to precipitate
after more than 24 hours incubation.  Before that, I can see no ppt
microscopically.  I wonder if the pH is an issue here because of that
observation.  In any event, the expected results still occur (i.e..
metaphase arrest with condensed chromosomes).


"Ian Harper" <Ian.Harper at sci.monash.edu.au> wrote in message
news:394F6E0C.C9F8791E at sci.monash.edu.au...
> Hi,
> A student of ours is using Taxol to disrupt the cytoskeleton, and has
> started getting unexpected preciptations forming in the medium after
> switching from a pharmaceutical grade to the "sigma" powder.  A stock is
> made up either in DMSO or Cremophor El (oil based I think) + Ethanol (
> as suggested by supplier), but when the stock is diluted 0.25% vol to
> the aqueous, we see needle-shaped crystal forming as taxol goes out of
> solution.
> Ideas, and protocols would be greatly appreciated,
> plz email to me: Ian.Harper at sci.monash.edu.au
>
> Thanks
>
> Ian.
>
> ><O><1><O><1><O><1><O><1><O><1><O><1><O><1><
> Ian Harper
> Confocal Imaging Facility
> Dept Biological Sciences
> Monash University
> Wellington Rd, Clayton
> VIC, Australia
> Tel: +61-3-99055635, Fax: +61-3-99055613
> Email: Ian.Harper at sci.monash.edu.au
> ><O><1><O><1><O><1><O><1><O><1><O><1><O><1><
>
>







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